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Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm & vesicles.
Antibody dilution
1:33
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA003263
Antibody HPA074818
Standard validation
Supported
Figure description
Immunohistochemical staining of human lymph node shows moderate nuclear positivity in reaction center cells and lymphoid cells outside reaction center.
Expression
RNA: detected in 37 tissues Protein: detected in 66 cell types
Retrieval
HIER pH6
Antibody dilution
1:5
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003263
Antibody HPA074818
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
68.9, 65.9, 65.6, 17.3
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA003263
Antibody HPA074818
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
