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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Antibody dilution
1:5
1:13
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA046386
Antibody HPA067203
Antibody CAB008094
Antibody CAB069409
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human prostate shows strong cytoplasmic, membranous and nuclear positivity in glandular cells.
Immunohistochemical staining of human parathyroid gland shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 27 cell types
RNA: detected in 37 tissues Protein: detected in 23 cell types
RNA: detected in 37 tissues Protein: detected in 61 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:600
1:50
1:1200
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA046386
Antibody HPA067203
Antibody CAB008094
Antibody CAB069409
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
