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PDIA3

ANTIBODY INFORMATION
 
 

Antibody HPA002645

  

Antibody HPA003230

  

Antibody CAB011199

  

Antibody CAB015181

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SIGMA
   Santa Cruz Biotechnology
  

Product name

 HPA002645   HPA003230   E5031   sc-28823  

Host species

 Rabbit   Rabbit   Mouse   Rabbit  

Clonality

 pAb   pAb   mAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G  

Other gene match

        

Released in version

 2   2   4   4  

References

 1   4      

VALIDATION SUMMARY
 
 

Antibody HPA002645

  

Antibody HPA003230

  

Antibody CAB011199

  

Antibody CAB015181

  
Immunocytochemistry


Validated
  

Validated

Validated
      
Immunohistochemistry


Validated
  

Validated
   Supported   Supported  
Western blot

Supported  

Validated
   Supported   Supported  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002645

  

Antibody HPA003230

  

Antibody CAB011199

  

Antibody CAB015181

  

Formal validation: Genetic

  
Validated

Significant downregulation > 25% by both siRNA:s.		      

 
 

Relative fluorescence intensity (RFI)

      

Figure description

   Whole cells of segmented cells in 10x-images      

Antibody dilution

   1:127      
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA003230.		  
Validated

Antibody staining overlaps with antibody HPA002645.		      
 

Standard validation

 Supported   Supported      

       

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.   Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.      

Antibody dilution

 1:20   1:125      

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002645

  

Antibody HPA003230

  

Antibody CAB011199

  

Antibody CAB015181

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		      

 

  
 

      

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA003230 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA002645 across 46 cell lines.		      
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 74 cell types		   RNA: detected in 37 tissues
Protein: detected in 63 cell types		   RNA: detected in 37 tissues
Protein: detected in 65 cell types		   RNA: detected in 37 tissues
Protein: detected in 62 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:6000   1:200   1:400  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002645

  

Antibody HPA003230

  

Antibody CAB011199

  

Antibody CAB015181

  

Formal validation: Genetic

  
Validated

Downregulation visible in one of two siRNA lanes		      

       

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		      

Target mass (kDa)

   56.8      

Loading control

   Total protein image      

Antibody dilution

   1:281      
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Liver
Lane 5: Tonsil
Lane 6: 		  Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 56.8   56.8   56.8   56.8  

Antibody dilution

 1:250   1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002645

  

Antibody HPA003230

  

Antibody CAB011199

  

Antibody CAB015181

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:2000   1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA002645

  

Antibody HPA003230

  

Antibody CAB011199

  

Antibody CAB015181

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Native protein   Recombinant protein  

Length (aa)

 132   118      

Antigen sequence

 
HMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGH
KLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRD
GKALERFLQDYFDGNLKRYLKSEPIPESNDGP
  
PTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFIS
DKDASIVGFFDDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNG
EGIILFRPSHLTNKFEDK
     

Matching transcripts

 PDIA3-001 - ENSP00000300289 [100%]
  PDIA3-001 - ENSP00000300289 [100%]
     

Other gene match

        
ANTIGEN VIEW
PDIA3-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA002645

  

Antibody HPA003230

  

Antibody CAB011199

  

Antibody CAB015181

  

1.

 SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome
Kashyap MK et al
Cancer Biol Ther 2010;10(8):796-810
   The involvement of SMILE/TMTC3 in endoplasmic reticulum stress response
Racapé M et al
PLoS One 2011;6(5):e19321
      

2.

   Loss-of-function mutations in MICU1 cause a brain and muscle disorder linked to primary alterations in mitochondrial calcium signaling
Logan CV et al
Nat Genet 2014;46(2):188-93
      

3.

   Proteomics based identification of cell migration related proteins in HBV expressing HepG2 cells
Feng H et al
PLoS One 2014;9(4):e95621
Application: WB 		     

4.

   The dehydrogenase region of the NADPH oxidase component Nox2 acts as a protein disulfide isomerase (PDI) resembling PDIA3 with a role in the binding of the activator protein p67 (phox.)
Bechor E et al
Front Chem 2015;3:3
Application: WB 		     
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.