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GOLGA2

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GENERAL INFORMATION

Gene name

 GOLGA2

Gene description

 Golgin A2

Protein class

 Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 7
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HUMAN PROTEIN ATLAS INFORMATION

Summary

 Mainly localized to the Golgi apparatus.

RNA cell category

 Expressed in all

Protein evidence

 Evidence at protein level

Main location

 Localized to the Golgi apparatus (validated)

siRNA

 The location(s) are Validated based on siRNA mediated gene silencing.

DATA RELIABILITY

Reliability score

 Validated based on 2 antibodies.
HPA021178 , HPA021799
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ASSAYS
RNA expression
Human cells
Custom data
siRNA

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Mainly localized to the Golgi apparatus.

Main location

 Golgi apparatus (validated)

 

 

 
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Low
High
G1
S
G2
M
N/A

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Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
HPA021178 A-431 Golgi apparatus
HPA021178 U-2 OS Golgi apparatus
HPA021178 U-251 MG Golgi apparatus
HPA021799 A-431 Golgi apparatus
HPA021799 U-2 OS Nucleus
Golgi apparatus
HPA021799 U-251 MG Nucleus
Golgi apparatus

CUSTOM DATA

 

 

 
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Antibody

Cell line

Assay

Description
HPA021178 U-2 OS Image Co-localization with the cis-Golgi marker GM130/GOLGA2 (RRID:AB_398141) after the treatment of U-2 OS cells with brefeldin A (5 ug/ml) for 30 minutes to disrupt the ER to Golgi transport; GOLGA2 localizes together with proteins associated with the Golgi-matrix (PMID: 8557739). GOLGA2 is a Golgi-matrix protein; HPA021178 and cis-Golgi marker GM130/GOLGA2 (Antibody RRID:AB_398141) validate each other, as they show similar stainings for the same target protein.
HPA021178 U-2 OS Image Co-localization with the cis-Golgi marker GM130/GOLGA2 (RRID:AB_398141) for a refined protein localization within the Golgi apparatus in U-2 OS cells. GOLGA2 localizes to the Golgi apparatus, and is only detected in the cis-Golgi compartment.
HPA021799 U-2 OS Image Co-localization with the cis-Golgi marker GM130/GOLGA2 (RRID:AB_398141) after the treatment of U-2 OS cells with brefeldin A (5 ug/ml) for 30 minutes to disrupt the ER to Golgi transport; GOLGA2 localizes together with proteins associated with the Golgi-matrix (PMID: 8557739). GOLGA2 is a Golgi-matrix protein; HPA021799 and cis-Golgi marker GM130/GOLGA2 (Antibody RRID:AB_398141) validate each other, as they show similar stainings for the same target protein.
HPA021799 U-2 OS Image Co-localization with the cis-Golgi marker GM130/GOLGA2 (RRID:AB_398141) for a refined protein localization within the Golgi apparatus in U-2 OS cells. GOLGA2 localizes to the Golgi apparatus, and is only detected in the cis-Golgi compartment.

siRNA VALIDATION
 

Relative fluorescence intensity (RFI)

Antibody

 HPA021799

siRNA validation

 Validated: Significant downregulation > 25% by both siRNA:s.

Analysis performed in

 Whole cells of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

 73%68%

p-value

 < 0.01< 0.01
 

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail 10x

Thumbnail 40x

Antibody

Sample

Cell line
HPA021799 siRNA 1 U-2 OS
HPA021799 siRNA 2 U-2 OS
HPA021799 scrambled U-2 OS

GENE INFORMATION

Gene name

 GOLGA2 (HGNC Symbol)

Synonyms

 GM130, golgin-95

Description

 Golgin A2 (HGNC Symbol)

Entrez gene summary

 The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010]

Chromosome

 9

Cytoband

 q34.11

Chromosome location (bp)

 128255829 - 128275995

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000167110 (version 83.38)

Entrez gene

 2801

UniProt

 Q08379 (UniProt - Evidence at protein level)

neXtProt

 NX_Q08379

Antibodypedia

 GOLGA2 antibodies

PROTEIN BROWSER
GOLGA2-004
GOLGA2-006
GOLGA2-008
GOLGA2-009
GOLGA2-014
GOLGA2-201
GOLGA2-202

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
GOLGA2-004
ENSP00000416097
ENST00000421699
Q08379 [Direct mapping]
Golgin subfamily A member 2
Show all
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000137 [Golgi cis cisterna]
GO:0000139 [Golgi membrane]
GO:0000278 [mitotic cell cycle]
GO:0000922 [spindle pole]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005794 [Golgi apparatus]
GO:0005801 [cis-Golgi network]
GO:0005874 [microtubule]
GO:0006486 [protein glycosylation]
GO:0006888 [ER to Golgi vesicle-mediated transport]
GO:0007020 [microtubule nucleation]
GO:0008017 [microtubule binding]
GO:0008356 [asymmetric cell division]
GO:0010507 [negative regulation of autophagy]
GO:0018279 [protein N-linked glycosylation via asparagine]
GO:0019901 [protein kinase binding]
GO:0019905 [syntaxin binding]
GO:0030134 [ER to Golgi transport vesicle]
GO:0032091 [negative regulation of protein binding]
GO:0032580 [Golgi cisterna membrane]
GO:0033116 [endoplasmic reticulum-Golgi intermediate compartment membrane]
GO:0043687 [post-translational protein modification]
GO:0044267 [cellular protein metabolic process]
GO:0048208 [COPII vesicle coating]
GO:0051225 [spindle assembly]
GO:0051289 [protein homotetramerization]
GO:0051297 [centrosome organization]
GO:0060050 [positive regulation of protein glycosylation]
GO:0061024 [membrane organization]
GO:0061676 [importin-alpha family protein binding]
GO:0072686 [mitotic spindle]
GO:0090161 [Golgi ribbon formation]
GO:0090166 [Golgi disassembly]
GO:0090306 [spindle assembly involved in meiosis]
GO:0090307 [mitotic spindle assembly]
Show all
 1002 aa
113.1 kDa 		No 0
GOLGA2-006
ENSP00000409271
ENST00000450617
B7ZC06 [Direct mapping]
Golgin subfamily A member 2
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005634 [nucleus]
GO:0005794 [Golgi apparatus]
Show all
 473 aa
53.4 kDa 		No 0
GOLGA2-008
ENSP00000473603
ENST00000490628
R4GND7 [Direct mapping]
Golgin subfamily A member 2
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005794 [Golgi apparatus]
Show all
 99 aa
10.7 kDa 		No 0
GOLGA2-009
ENSP00000411598
ENST00000458730
H0Y7B8 [Direct mapping]
Golgin subfamily A member 2
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005794 [Golgi apparatus]
Show all
 235 aa
25.9 kDa 		No 0
GOLGA2-014
ENSP00000476265
ENST00000609374
A0A0C4DGS5 [Direct mapping]
Golgi autoantigen, golgin subfamily a, 2, isoform CRA_a; Golgin subfamily A member 2
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005794 [Golgi apparatus]
GO:0005801 [cis-Golgi network]
Show all
 990 aa
111.7 kDa 		No 0
GOLGA2-201
ENSP00000481685
ENST00000610329
A0A087WYC0 [Direct mapping]
Golgin subfamily A member 2
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005794 [Golgi apparatus]
Show all
 286 aa
31.9 kDa 		No 0
GOLGA2-202
ENSP00000478799
ENST00000611957
Q08379 [Direct mapping]
Golgin subfamily A member 2
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000137 [Golgi cis cisterna]
GO:0000139 [Golgi membrane]
GO:0000278 [mitotic cell cycle]
GO:0000922 [spindle pole]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005794 [Golgi apparatus]
GO:0005801 [cis-Golgi network]
GO:0005874 [microtubule]
GO:0006486 [protein glycosylation]
GO:0006888 [ER to Golgi vesicle-mediated transport]
GO:0007020 [microtubule nucleation]
GO:0008017 [microtubule binding]
GO:0008356 [asymmetric cell division]
GO:0010507 [negative regulation of autophagy]
GO:0018279 [protein N-linked glycosylation via asparagine]
GO:0019901 [protein kinase binding]
GO:0019905 [syntaxin binding]
GO:0030134 [ER to Golgi transport vesicle]
GO:0032091 [negative regulation of protein binding]
GO:0032580 [Golgi cisterna membrane]
GO:0033116 [endoplasmic reticulum-Golgi intermediate compartment membrane]
GO:0043687 [post-translational protein modification]
GO:0044267 [cellular protein metabolic process]
GO:0048208 [COPII vesicle coating]
GO:0051225 [spindle assembly]
GO:0051289 [protein homotetramerization]
GO:0051297 [centrosome organization]
GO:0060050 [positive regulation of protein glycosylation]
GO:0061024 [membrane organization]
GO:0061676 [importin-alpha family protein binding]
GO:0072686 [mitotic spindle]
GO:0090161 [Golgi ribbon formation]
GO:0090166 [Golgi disassembly]
GO:0090306 [spindle assembly involved in meiosis]
GO:0090307 [mitotic spindle assembly]
Show all
 1002 aa
113.1 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.