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ISLR2

ANTIBODY INFORMATION
 
 

Antibody HPA012930

  

Antibody HPA067333

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA012930   HPA067333  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 14   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA012930

  

Antibody HPA067333

  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA012930

  

Antibody HPA067333

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.   Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.  

Expression

 RNA: detected in 15 tissues
Protein: detected in 38 cell types		   RNA: detected in 15 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:3000  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA012930

  

Antibody HPA067333

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 79   79, 36.2  

Antibody dilution

 1:250   1:670  

PROTEIN ARRAY
 
 

Antibody HPA012930

  

Antibody HPA067333

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:8100  

ANTIGEN INFORMATION
 
 

Antibody HPA012930

  

Antibody HPA067333

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 117   89  

Antigen sequence

 
GLAFVLHCIADGHPTPRLQWQLQIPGGTVVLEPPVLSGEDDGVGAEEGEG
EGDGDLLTQTQAQTPTPAPAWPAPPATPRFLALANGSLLVPLLSAKEAGV
YTCRAHNELGANSTSIR
  
ESEKSYPAGGEAGGEEPEDVQGEGLDEDAEQGDPSGDLQREESLAACSLV
ESQSKANQEEFEAGSEYSDRLPLGAEAVNIAQEINGNYR
 

Matching transcripts

 ISLR2-001 - ENSP00000355402 [100%]
ISLR2-004 - ENSP00000455531 [100%]
ISLR2-005 - ENSP00000411834 [100%]
ISLR2-007 - ENSP00000458080 [100%]
ISLR2-202 - ENSP00000411443 [100%]
  ISLR2-001 - ENSP00000355402 [100%]
ISLR2-004 - ENSP00000455531 [100%]
ISLR2-005 - ENSP00000411834 [100%]
ISLR2-007 - ENSP00000458080 [100%]
ISLR2-201 - ENSP00000408872 [100%]
ISLR2-202 - ENSP00000411443 [100%]
 

Other gene match

    
ANTIGEN VIEW
ISLR2-001
ISLR2-004
ISLR2-005
ISLR2-007
ISLR2-201
ISLR2-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.