RPL13 - Antibodies - The Human Protein Atlas

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RPL13

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA051702

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA051702

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA051702

Immunocytochemistry

Immunohistochemistry

Approved

Western blot

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA051702

Formal validation: Genetic

Validated

Significant downregulation > 25% by one siRNA.

Relative fluorescence intensity (RFI)

Figure description

Cytoplasmic region of segmented cells in 10x-images

Antibody dilution

1:6

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli, cytosol & endoplasmic reticulum.

Antibody dilution

1:6

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA051702

Standard validation

Approved

Figure description

Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 67 cell types

Retrieval

HIER pH6

Antibody dilution

1:20

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA051702

Formal validation: Genetic

Validated

Downregulation visible in both siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

24.3, 18.8

Loading control

Total protein image

Antibody dilution

1:58

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

24.3, 18.8

Antibody dilution

1:80

PROTEIN ARRAY

Antibody HPA051702

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

ANTIGEN INFORMATION

Antibody HPA051702

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

PKKGDSSAEELKLATQLTGPVMPVRNVYKKEKARVITEEEKNFKAFASLR
MARANARLFGIRAKRAKEAAEQD

Matching transcripts

RPL13-001 - ENSP00000307889 [100%]
RPL13-002 - ENSP00000376811 [100%]
RPL13-009 - ENSP00000455009 [100%]
RPL13-015 - ENSP00000438959 [99%]

Other gene match

ANTIGEN VIEW

RPL13-001	RPL13-002	RPL13-009	RPL13-015

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.