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Immunohistochemical staining of human cerebral cortex shows strong nuclear membrane positivity in neuronal cells.
Expression
RNA: detected in 32 tissues Protein: detected in 57 cell types
Retrieval
HIER pH6
Antibody dilution
1:35
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA055771
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
53.5, 48.7
Antibody dilution
1:80
PROTEIN ARRAY
Antibody HPA055771
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
