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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Antibody dilution
1:76
1:8
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA042004
Antibody HPA042056
Antibody HPA061571
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human spleen shows moderate cytoplasmic positivity in red and white pulp.
Expression
RNA: detected in 37 tissues Protein: detected in 15 cell types
Retrieval
HIER pH6
Antibody dilution
1:450
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA042004
Antibody HPA042056
Antibody HPA061571
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409383)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409383)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
95.5, 91.7, 88.2, 86.3, 32.3
95.5, 91.7, 88.2, 86.3
95.5, 91.7, 88.2, 86.3
Antibody dilution
1:250
1:250
1:80
PROTEIN ARRAY
Antibody HPA042004
Antibody HPA042056
Antibody HPA061571
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
