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ZNF526

ANTIBODY INFORMATION
 
 

Antibody HPA023070

  

Antibody HPA056609

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA023070   HPA056609  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA023070

  

Antibody HPA056609

  
Immunocytochemistry

Approved    
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Supported   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA023070

  

Antibody HPA056609

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line A549 shows localization to nucleus & the Golgi apparatus.    

Antibody dilution

 1:149    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA023070

  

Antibody HPA056609

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in dot like pattern in glandular cells.   Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 58 cell types		   RNA: detected in 35 tissues
Protein: detected in 60 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:1100  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA023070

  

Antibody HPA056609

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 73.6   73.6, 7.5  

Antibody dilution

 1:250   1:470  

PROTEIN ARRAY
 
 

Antibody HPA023070

  

Antibody HPA056609

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:5600  

ANTIGEN INFORMATION
 
 

Antibody HPA023070

  

Antibody HPA056609

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 75   78  

Antigen sequence

 
GLEEEEEDDEEDEEDDEEMEDEEAMAEVGDDAVGGDESTAGWAQGCGDCP
QHQPSAGARRQHRRTAHSPASATHP
  
AEVAEMPTQMSPGAVEMSTPMSAEMMEMSTEVTEMTPGEALASSLFFQHH
QFMCSECGSLYNTLEEVLSHQEQHMLAV
 

Matching transcripts

 ZNF526-001 - ENSP00000301215 [100%]
  ZNF526-001 - ENSP00000301215 [100%]
ZNF526-003 - ENSP00000473075 [82%]
 

Other gene match

    
ANTIGEN VIEW
ZNF526-001
ZNF526-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.