We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to cytosol & mitochondria.
Antibody dilution
1:82
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA045211
Antibody HPA075051
Standard validation
Approved
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 43 cell types
Retrieval
HIER pH6
Antibody dilution
1:20
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA045211
Antibody HPA075051
Standard validation
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416198)
Target mass (kDa)
50.8, 50.1, 49.9, 19.8, 19.7, 15.6
Antibody dilution
1:125
PROTEIN ARRAY
Antibody HPA045211
Antibody HPA075051
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
