CTNNB1 - Antibodies - The Human Protein Atlas

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CTNNB1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA029159

Antibody HPA029160

Antibody CAB000108

Antibody CAB001950

Provider

Atlas Antibodies
Sigma-Aldrich

Chemicon

Upstate

Product name

HPA029159

HPA029160

AB19022

05-665

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA029159

Antibody HPA029160

Antibody CAB000108

Antibody CAB001950

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA029159

Antibody HPA029160

Antibody CAB000108

Antibody CAB001950

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA029160.

Validated

Antibody staining overlaps with antibody HPA029159.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

Antibody dilution

1:100

1:50

1:19

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA029159

Antibody HPA029160

Antibody CAB000108

Antibody CAB001950

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA029160 across 45 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA029159 across 45 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA029160 across 72 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA029159 across 72 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.

Immunohistochemical staining of human liver shows strong positivity of cytoplasm and nuclei in hepatocytes.

Expression

RNA: detected in 37 tissues
Protein: detected in 72 cell types

RNA: detected in 37 tissues
Protein: detected in 59 cell types

RNA: detected in 37 tissues
Protein: detected in 65 cell types

RNA: detected in 37 tissues
Protein: detected in 28 cell types

Retrieval

HIER pH6

Antibody dilution

1:600

1:250

1:75

1:50

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA029159

Antibody HPA029160

Antibody CAB000108

Antibody CAB001950

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

85.5, 84.8, 16.6, 12, 11.1

85.5, 84.8

85.5, 84.8, 16.6, 12, 11.1, 6.5, 1.4

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA029159

Antibody HPA029160

Antibody CAB000108

Antibody CAB001950

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA029159

Antibody HPA029160

Antibody CAB000108

Antibody CAB001950

Antigen

Recombinant protein fragment

Not known

Length (aa)

Antigen sequence

SYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQ
VADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAH

SEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQD
DPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPD

Matching transcripts

CTNNB1-001 - ENSP00000344456 [100%]
CTNNB1-002 - ENSP00000379488 [100%]
CTNNB1-003 - ENSP00000379486 [100%]
CTNNB1-005 - ENSP00000411226 [100%]
CTNNB1-007 - ENSP00000385604 [100%]
CTNNB1-012 - ENSP00000412219 [100%]
CTNNB1-013 - ENSP00000409302 [89%]
CTNNB1-014 - ENSP00000401599 [80%]

CTNNB1-001 - ENSP00000344456 [100%]
CTNNB1-002 - ENSP00000379488 [100%]
CTNNB1-003 - ENSP00000379486 [100%]
CTNNB1-005 - ENSP00000411226 [100%]
CTNNB1-007 - ENSP00000385604 [100%]

Other gene match

ANTIGEN VIEW

CTNNB1-001	CTNNB1-002	CTNNB1-003	CTNNB1-005	CTNNB1-007	CTNNB1-012	CTNNB1-013	CTNNB1-014

RELEVANT PUBLICATIONS

Antibody HPA029159

Antibody HPA029160

Antibody CAB000108

Antibody CAB001950

The Urotensin II System and Carotid Atherosclerosis: A Role in Vascular Calcification
Albanese I et al
Front Pharmacol 2016;7:149

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.