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POLR2J3

ANTIBODY INFORMATION
 
 

Antibody HPA011403

  

Antibody HPA042997

  

Antibody HPA044010

  

Antibody HPA046970

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA011403   HPA042997   HPA044010   HPA046970  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 UPK3BL - ENSG00000267368 [99%]
   POLR2J - ENSG00000005075 [98%]
POLR2J2 - ENSG00000228049 [100%]
   POLR2J - ENSG00000005075 [100%]
POLR2J2 - ENSG00000228049 [100%]
   POLR2J - ENSG00000005075 [100%]
POLR2J2 - ENSG00000228049 [97%]
RP11-514P8.7 - ENSG00000270249 [97%]
  

Released in version

 11   11   11   11  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA011403

  

Antibody HPA042997

  

Antibody HPA044010

  

Antibody HPA046970

  
Immunocytochemistry

      Approved  
Immunohistochemistry

Approved   Approved   Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA011403

  

Antibody HPA042997

  

Antibody HPA044010

  

Antibody HPA046970

  

Standard validation

       Approved  

       

Figure description

       Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

       1:43  

Literature conformity

       The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA011403

  

Antibody HPA042997

  

Antibody HPA044010

  

Antibody HPA046970

  

Standard validation

 Approved   Approved   Approved   Approved  

       

Figure description

 Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.   Immunohistochemical staining of human salivary gland shows strong cytoplasmic positivity in red blood cells.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in bile duct cells.   Immunohistochemical staining of human liver shows moderate cytoplasmic and nuclear positivity in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 19 cell types		   RNA: detected in 37 tissues
Protein: detected in 3 cell types		   RNA: detected in 37 tissues
Protein: detected in 15 cell types		   RNA: detected in 37 tissues
Protein: detected in 28 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:25   1:75   1:120  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA011403

  

Antibody HPA042997

  

Antibody HPA044010

  

Antibody HPA046970

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

       

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 30.4, 28.4   17.2, 17.1   17.2, 17.1, 13.3, 13.1   30.9, 30.4, 17.2, 17.1, 13.3, 13.1, 8.4, 8  

Antibody dilution

 1:250   1:250   1:250   1:210  

PROTEIN ARRAY
 
 

Antibody HPA011403

  

Antibody HPA042997

  

Antibody HPA044010

  

Antibody HPA046970

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:3000   1:1700  

ANTIGEN INFORMATION
 
 

Antibody HPA011403

  

Antibody HPA042997

  

Antibody HPA044010

  

Antibody HPA046970

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 137   43   54   31  

Antigen sequence

 
VEHISYVPQLSNDTLAGRLTLSTFTLEQPLGQFSSHNISDLDTIWLVVAL
SNATQSFTAPRTNQDIPAPANFSQRGYYLTLRANRVLYQTRGQLHVLRVG
NDTHCQPTKIGCNHPLPGPGPYRVKFLVMNDEGPVAE
  
VGWTLARVPRPGTALACFFGGPQGEAAVMEEQGLPPQAPGHVD
  
DPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLE
ERFR
  
KITINKDTKVPNACLFTINKEDHTLGNIIKS
 

Matching transcripts

 POLR2J3-010 - ENSP00000421085 [99%]
  POLR2J3-201 - ENSP00000482932 [100%]
  POLR2J3-020 - ENSP00000483328 [100%]
POLR2J3-201 - ENSP00000482932 [100%]
  POLR2J3-005 - ENSP00000422109 [100%]
POLR2J3-010 - ENSP00000421085 [100%]
POLR2J3-012 - ENSP00000421950 [100%]
POLR2J3-020 - ENSP00000483328 [100%]
POLR2J3-201 - ENSP00000482932 [100%]
 

Other gene match

 UPK3BL - ENSG00000267368 [99%]
   POLR2J - ENSG00000005075 [98%]
POLR2J2 - ENSG00000228049 [100%]
   POLR2J - ENSG00000005075 [100%]
POLR2J2 - ENSG00000228049 [100%]
   POLR2J - ENSG00000005075 [100%]
POLR2J2 - ENSG00000228049 [97%]
RP11-514P8.7 - ENSG00000270249 [97%]
  
ANTIGEN VIEW
POLR2J3-005
POLR2J3-010
POLR2J3-012
POLR2J3-020
POLR2J3-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.