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XIRP1

ANTIBODY INFORMATION
 
 

Antibody HPA016750

  

Antibody HPA018458

  

Antibody CAB026391

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA016750   HPA018458   3583.00.02  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 5   5   6  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA016750

  

Antibody HPA018458

  

Antibody CAB026391

  
Immunocytochemistry

    Uncertain  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA016750

  

Antibody HPA018458

  

Antibody CAB026391

  

Standard validation

     Uncertain  

     

Figure description

     Immunofluorescent staining of human cell line A-431 shows localization to nucleus & actin filaments.  

Antibody dilution

     1:100  

Literature conformity

     The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA016750

  

Antibody HPA018458

  

Antibody CAB026391

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.   Immunohistochemical staining of human heart muscle shows intercalated disc positivity in myocytes.   Immunohistochemical staining of human heart muscle shows strong positivity in intercalated discs.  

Expression

 RNA: detected in 8 tissues
Protein: detected in 10 cell types		   RNA: detected in 8 tissues
Protein: detected in 26 cell types		   RNA: detected in 8 tissues
Protein: detected in 2 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:1100   1:75   1:400  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA016750

  

Antibody HPA018458

  

Antibody CAB026391

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 198.6, 122.1   198.6, 56.3   198.6, 122.1, 56.3  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA016750

  

Antibody HPA018458

  

Antibody CAB026391

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA016750

  

Antibody HPA018458

  

Antibody CAB026391

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Genetic immunization  

Length (aa)

 106   101    

Antigen sequence

 
QSCTWMFKPQPVDRPVGSREQHLQVSQVPAGERQTDRHVFETEPLQASGR
PCGRRPVRYCSRVEIPSGQVSRQKEVFQALEAGKKEEQEPRVIAGSIPAG
SVHKFT
  
RVSTEVAQLKEQTLARLLDIEEAVHKALSSMSSLQPEASARGHFQGPPKD
HSAHKISVTVSSSARPSGSGQEVGGQTAVKNQAKVECHTEAQSQVKIRNH
T
  

											 
																				

																		

										
											
Matching transcripts

												
																					XIRP1-001 - ENSP00000343140 [100%] 
XIRP1-002 - ENSP00000379550 [100%] 

											 
																						XIRP1-001 - ENSP00000343140 [100%] 
XIRP1-004 - ENSP00000391645 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
XIRP1-001
XIRP1-002
XIRP1-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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