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TRAPPC11

ANTIBODY INFORMATION
 
 

Antibody HPA045427

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA045427  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 10  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA045427

  
Immunocytochemistry

Approved  
Western blot

Supported  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA045427

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear membrane.  

Antibody dilution

 1:46  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA045427

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 128.9, 123.4  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA045427

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA045427

  

Antigen

 Recombinant protein fragment  

Length (aa)

 75  

Antigen sequence

 
YELDWDEPQWKEKQSECATRVEIVRQSLQGRNTKVAVVLIQKKTPLPPGE
DVIASERAAALCNACELSGKSLFVL
 

Matching transcripts

 TRAPPC11-001 - ENSP00000335371 [100%]
TRAPPC11-201 - ENSP00000349738 [100%]
 

Other gene match

  
ANTIGEN VIEW
TRAPPC11-001
TRAPPC11-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.