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Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & vesicles.
Antibody dilution
1:85
1:7
Literature conformity
The subcellular location is not consistent with literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA047633
Antibody HPA054556
Antibody HPA065246
Standard validation
Approved
Figure description
Immunohistochemical staining of human lymph node shows moderate nuclear positivity in germinal center cells and non-germinal center cells.
Expression
RNA: detected in 36 tissues Protein: detected in 78 cell types
Retrieval
HIER pH6
Antibody dilution
1:2300
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA047633
Antibody HPA054556
Antibody HPA065246
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
33.9, 32.1, 30.1, 28.2, 25.6, 23.1
33.9, 30.1, 25.6
33.9, 30.1, 25.6
Antibody dilution
1:420
1:80
1:1010
PROTEIN ARRAY
Antibody HPA047633
Antibody HPA054556
Antibody HPA065246
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
