Cell atlas - MFF - The Human Protein Atlas

GENERAL INFORMATION
Gene name	MFF
Gene description	Mitochondrial fission factor
Protein class	Predicted intracellular proteins Predicted membrane proteins
Predicted localization	Intracellular,Membrane
Number of transcripts	19
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Localized to the mitochondria.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Mitochondria (supported)
Single-cell variation	Single-cell variation in protein expression observed.

DATA RELIABILITY
Reliability score	Supported based on 1 antibody. HPA074625
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ASSAYS

RNA expression

Human cells

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Localized to the mitochondria.

Main location

Mitochondria (supported)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location	Single-cell variation
HPA074625	HEK 293	Mitochondria
HPA074625	SiHa	Mitochondria	Intensity
HPA074625	U-2 OS	Mitochondria

GENE INFORMATION
Gene name	MFF (HGNC Symbol)
Synonyms	C2orf33, GL004
Description	Mitochondrial fission factor (HGNC Symbol)
Entrez gene summary	This is a nuclear gene encoding a protein that functions in mitochondrial and peroxisomal fission. The encoded protein recruits dynamin-1-like protein (DNM1L) to mitochondria. There are multiple pseudogenes for this gene on chromosomes 1, 5, and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
Chromosome	2
Cytoband	q36.3
Chromosome location (bp)	227325151 - 227357836
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000168958 (version 83.38)
Entrez gene	56947
UniProt	Q9GZY8 (UniProt - Evidence at protein level)
neXtProt	NX_Q9GZY8
Antibodypedia	MFF antibodies

PROTEIN BROWSER

MFF-001	MFF-002	MFF-003	MFF-005	MFF-008	MFF-010	MFF-013	MFF-016	MFF-017	MFF-018	MFF-019	MFF-021	MFF-022	MFF-023	MFF-024	MFF-025	MFF-026	MFF-027	MFF-201

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
MFF-001 ENSP00000302037 ENST00000353339	Q9GZY8 [Direct mapping] Mitochondrial fission factor A0A024R491 [Target identity:100%; Query identity:100%] Chromosome 2 open reading frame 33, isoform CRA_a Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000266 [mitochondrial fission] GO:0001836 [release of cytochrome c from mitochondria] GO:0005515 [protein binding] GO:0005739 [mitochondrion] GO:0005741 [mitochondrial outer membrane] GO:0005777 [peroxisome] GO:0006626 [protein targeting to mitochondrion] GO:0008021 [synaptic vesicle] GO:0008053 [mitochondrial fusion] GO:0010821 [regulation of mitochondrion organization] GO:0016559 [peroxisome fission] GO:0030054 [cell junction] GO:0032592 [integral component of mitochondrial membrane] GO:0042803 [protein homodimerization activity] GO:0043653 [mitochondrial fragmentation involved in apoptotic process] GO:0051260 [protein homooligomerization] GO:0070584 [mitochondrion morphogenesis] GO:0090141 [positive regulation of mitochondrial fission] GO:0090200 [positive regulation of release of cytochrome c from mitochondria] GO:0090314 [positive regulation of protein targeting to membrane] GO:1900063 [regulation of peroxisome organization] Show all	342 aa 38.5 kDa	No	1
MFF-002 ENSP00000338412 ENST00000337110	Q9GZY8 [Direct mapping] Mitochondrial fission factor Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Ezkurdia et al 2014) Show all	GO:0000266 [mitochondrial fission] GO:0001836 [release of cytochrome c from mitochondria] GO:0005515 [protein binding] GO:0005741 [mitochondrial outer membrane] GO:0005777 [peroxisome] GO:0006626 [protein targeting to mitochondrion] GO:0008021 [synaptic vesicle] GO:0008053 [mitochondrial fusion] GO:0010821 [regulation of mitochondrion organization] GO:0016559 [peroxisome fission] GO:0030054 [cell junction] GO:0032592 [integral component of mitochondrial membrane] GO:0042803 [protein homodimerization activity] GO:0043653 [mitochondrial fragmentation involved in apoptotic process] GO:0051260 [protein homooligomerization] GO:0070584 [mitochondrion morphogenesis] GO:0090141 [positive regulation of mitochondrial fission] GO:0090200 [positive regulation of release of cytochrome c from mitochondria] GO:0090314 [positive regulation of protein targeting to membrane] GO:1900063 [regulation of peroxisome organization] Show all	243 aa 28 kDa	No	1
MFF-003 ENSP00000304898 ENST00000304593	Q9GZY8 [Direct mapping] Mitochondrial fission factor Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Ezkurdia et al 2014) Show all	GO:0000266 [mitochondrial fission] GO:0001836 [release of cytochrome c from mitochondria] GO:0005515 [protein binding] GO:0005741 [mitochondrial outer membrane] GO:0005777 [peroxisome] GO:0006626 [protein targeting to mitochondrion] GO:0008021 [synaptic vesicle] GO:0008053 [mitochondrial fusion] GO:0010821 [regulation of mitochondrion organization] GO:0016559 [peroxisome fission] GO:0030054 [cell junction] GO:0032592 [integral component of mitochondrial membrane] GO:0042803 [protein homodimerization activity] GO:0043653 [mitochondrial fragmentation involved in apoptotic process] GO:0051260 [protein homooligomerization] GO:0070584 [mitochondrion morphogenesis] GO:0090141 [positive regulation of mitochondrial fission] GO:0090200 [positive regulation of release of cytochrome c from mitochondria] GO:0090314 [positive regulation of protein targeting to membrane] GO:1900063 [regulation of peroxisome organization] Show all	291 aa 33 kDa	No	1
MFF-005 ENSP00000386964 ENST00000409565	Q9GZY8 [Direct mapping] Mitochondrial fission factor Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Ezkurdia et al 2014) Show all	GO:0000266 [mitochondrial fission] GO:0001836 [release of cytochrome c from mitochondria] GO:0005515 [protein binding] GO:0005741 [mitochondrial outer membrane] GO:0005777 [peroxisome] GO:0006626 [protein targeting to mitochondrion] GO:0008021 [synaptic vesicle] GO:0008053 [mitochondrial fusion] GO:0010821 [regulation of mitochondrion organization] GO:0016559 [peroxisome fission] GO:0030054 [cell junction] GO:0032592 [integral component of mitochondrial membrane] GO:0042803 [protein homodimerization activity] GO:0043653 [mitochondrial fragmentation involved in apoptotic process] GO:0051260 [protein homooligomerization] GO:0070584 [mitochondrion morphogenesis] GO:0090141 [positive regulation of mitochondrial fission] GO:0090200 [positive regulation of release of cytochrome c from mitochondria] GO:0090314 [positive regulation of protein targeting to membrane] GO:1900063 [regulation of peroxisome organization] Show all	218 aa 25.1 kDa	No	1
MFF-008 ENSP00000386641 ENST00000409616	Q9GZY8 [Direct mapping] Mitochondrial fission factor Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Ezkurdia et al 2014) Show all	GO:0000266 [mitochondrial fission] GO:0001836 [release of cytochrome c from mitochondria] GO:0005515 [protein binding] GO:0005741 [mitochondrial outer membrane] GO:0005777 [peroxisome] GO:0006626 [protein targeting to mitochondrion] GO:0008021 [synaptic vesicle] GO:0008053 [mitochondrial fusion] GO:0010821 [regulation of mitochondrion organization] GO:0016559 [peroxisome fission] GO:0030054 [cell junction] GO:0032592 [integral component of mitochondrial membrane] GO:0042803 [protein homodimerization activity] GO:0043653 [mitochondrial fragmentation involved in apoptotic process] GO:0051260 [protein homooligomerization] GO:0070584 [mitochondrion morphogenesis] GO:0090141 [positive regulation of mitochondrial fission] GO:0090200 [positive regulation of release of cytochrome c from mitochondria] GO:0090314 [positive regulation of protein targeting to membrane] GO:1900063 [regulation of peroxisome organization] Show all	238 aa 27.1 kDa	No	1
MFF-010 ENSP00000390165 ENST00000423098	C9JI76 [Direct mapping] Mitochondrial fission factor Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		32 aa 3.8 kDa	No	0
MFF-013 ENSP00000411386 ENST00000436237	C9J846 [Direct mapping] Mitochondrial fission factor Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		34 aa 3.5 kDa	No	0
MFF-016 ENSP00000391829 ENST00000443428	C9JAF1 [Direct mapping] Mitochondrial fission factor Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		71 aa 7.8 kDa	No	0
MFF-017 ENSP00000415996 ENST00000452930	C9JHF5 [Direct mapping] Mitochondrial fission factor Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		138 aa 15.6 kDa	No	0
MFF-018 ENSP00000407547 ENST00000418961	C9JU19 [Direct mapping] Mitochondrial fission factor Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		60 aa 6.8 kDa	No	0
MFF-019 ENSP00000415313 ENST00000456345	H7C433 [Direct mapping] Mitochondrial fission factor Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Ezkurdia et al 2014) Show all	GO:0016021 [integral component of membrane] Show all	154 aa 17.8 kDa	No	1
MFF-021 ENSP00000304134 ENST00000349901	Q9GZY8 [Direct mapping] Mitochondrial fission factor Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Ezkurdia et al 2014) Show all	GO:0000266 [mitochondrial fission] GO:0001836 [release of cytochrome c from mitochondria] GO:0005515 [protein binding] GO:0005741 [mitochondrial outer membrane] GO:0005777 [peroxisome] GO:0006626 [protein targeting to mitochondrion] GO:0008021 [synaptic vesicle] GO:0008053 [mitochondrial fusion] GO:0010821 [regulation of mitochondrion organization] GO:0016559 [peroxisome fission] GO:0030054 [cell junction] GO:0032592 [integral component of mitochondrial membrane] GO:0042803 [protein homodimerization activity] GO:0043653 [mitochondrial fragmentation involved in apoptotic process] GO:0051260 [protein homooligomerization] GO:0070584 [mitochondrion morphogenesis] GO:0090141 [positive regulation of mitochondrial fission] GO:0090200 [positive regulation of release of cytochrome c from mitochondria] GO:0090314 [positive regulation of protein targeting to membrane] GO:1900063 [regulation of peroxisome organization] Show all	238 aa 27.1 kDa	No	1
MFF-022 ENSP00000346498 ENST00000354503	Q9GZY8 [Direct mapping] Mitochondrial fission factor Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Ezkurdia et al 2014) Show all	GO:0000266 [mitochondrial fission] GO:0001836 [release of cytochrome c from mitochondria] GO:0005515 [protein binding] GO:0005741 [mitochondrial outer membrane] GO:0005777 [peroxisome] GO:0006626 [protein targeting to mitochondrion] GO:0008021 [synaptic vesicle] GO:0008053 [mitochondrial fusion] GO:0010821 [regulation of mitochondrion organization] GO:0016559 [peroxisome fission] GO:0030054 [cell junction] GO:0032592 [integral component of mitochondrial membrane] GO:0042803 [protein homodimerization activity] GO:0043653 [mitochondrial fragmentation involved in apoptotic process] GO:0051260 [protein homooligomerization] GO:0070584 [mitochondrion morphogenesis] GO:0090141 [positive regulation of mitochondrial fission] GO:0090200 [positive regulation of release of cytochrome c from mitochondria] GO:0090314 [positive regulation of protein targeting to membrane] GO:1900063 [regulation of peroxisome organization] Show all	218 aa 25.1 kDa	No	1
MFF-023 ENSP00000431725 ENST00000530359	E9PK16 [Direct mapping] Mitochondrial fission factor Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		59 aa 6.7 kDa	No	0
MFF-024 ENSP00000432447 ENST00000531278	E9PQX8 [Direct mapping] Mitochondrial fission factor Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		69 aa 7.6 kDa	No	0
MFF-025 ENSP00000436920 ENST00000525195	E9PPR7 [Direct mapping] Mitochondrial fission factor Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		52 aa 5.9 kDa	No	0
MFF-026 ENSP00000435354 ENST00000534203	E9PKS0 [Direct mapping] Mitochondrial fission factor Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		71 aa 7.9 kDa	No	0
MFF-027 ENSP00000436289 ENST00000524634	E9PPW6 [Direct mapping] Mitochondrial fission factor Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Ezkurdia et al 2014) Show all	GO:0016021 [integral component of membrane] Show all	89 aa 10.5 kDa	No	1
MFF-201 ENSP00000375912 ENST00000392059	A0A0A0MS29 [Direct mapping] Mitochondrial fission factor Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Ezkurdia et al 2014) Show all	GO:0016021 [integral component of membrane] Show all	221 aa 25.3 kDa	No	1