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Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.
Antibody dilution
1:25
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA018994
Antibody HPA062126
Antibody CAB026342
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human skin shows cytoplasmic positivity in keratinocytes.
Immunohistochemical staining of human skin shows cytoplasmic positivity in keratinocytes.
Expression
RNA: detected in 11 tissues Protein: detected in 11 cell types
RNA: detected in 11 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1200
1:600
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA018994
Antibody HPA062126
Antibody CAB026342
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417538)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417538)
Target mass (kDa)
27.5, 19.9
27.5, 19.9, 7.8
27.5, 19.9, 7.8
Antibody dilution
1:250
1:125
1:500
PROTEIN ARRAY
Antibody HPA018994
Antibody HPA062126
Antibody CAB026342
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
