SDC2 - Antibodies - The Human Protein Atlas

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SDC2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA044416

Antibody CAB025123

Provider

Atlas Antibodies
Sigma-Aldrich

R&D Systems

Product name

HPA044416

MAB29651

Host species

Rabbit

Rat

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA044416

Antibody CAB025123

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA044416

Antibody CAB025123

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line Hep G2 shows localization to plasma membrane & cytosol.

Antibody dilution

1:200

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA044416

Antibody CAB025123

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human prostate shows strong cytoplasmic positivity with a granular pattern in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 76 cell types

Retrieval

HIER pH6

Antibody dilution

1:75

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA044416

Antibody CAB025123

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Target mass (kDa)

22.2, 19, 18.4, 16, 14.9

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA044416

Antibody CAB025123

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA044416

Antibody CAB025123

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

KVETTTLNIQNKIPAQTKSPEETDKEKVHLSDSERKMDPAEEDTNVYTEK
HSDSLFKRTEVL

Matching transcripts

SDC2-001 - ENSP00000307046 [100%]
SDC2-002 - ENSP00000429045 [100%]
SDC2-003 - ENSP00000428256 [100%]
SDC2-004 - ENSP00000429121 [100%]
SDC2-005 - ENSP00000429662 [100%]
SDC2-006 - ENSP00000427784 [100%]

Other gene match

ANTIGEN VIEW

SDC2-001	SDC2-002	SDC2-003	SDC2-004	SDC2-005	SDC2-006

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.