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Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & the Golgi apparatus.
Antibody dilution
1:44
1:182
Literature conformity
The subcellular location is not consistent with literature.
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA041995
Antibody HPA042988
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA042988 across 43 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA041995 across 43 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA042988 across 73 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA041995 across 73 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Expression
RNA: detected in 37 tissues Protein: detected in 58 cell types
RNA: detected in 37 tissues Protein: detected in 43 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:100
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA041995
Antibody HPA042988
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY428102)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY428102)
Target mass (kDa)
61.1, 57.2, 56.6, 51.3, 49, 19
61.1, 56.6, 51.3, 49.1, 49, 19.6
Antibody dilution
1:125
1:250
PROTEIN ARRAY
Antibody HPA041995
Antibody HPA042988
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
