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FASN

ANTIBODY INFORMATION
 
 

Antibody HPA006461

  

Antibody HPA056108

  

Antibody CAB005192

  

Antibody CAB015417

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Santa Cruz Biotechnology
  

Product name

 HPA006461   HPA056108   sc-20140   sc-55580  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

        

Released in version

 4   14   3   4  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA006461

  

Antibody HPA056108

  

Antibody CAB005192

  

Antibody CAB015417

  
Immunocytochemistry

Supported     Supported    
Immunohistochemistry

Supported   Supported   Supported  

Validated
  
Western blot

Supported   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA006461

  

Antibody HPA056108

  

Antibody CAB005192

  

Antibody CAB015417

  

Standard validation

 Supported     Supported    

       

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.     Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.    

Antibody dilution

 1:20     1:19    

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006461

  

Antibody HPA056108

  

Antibody CAB005192

  

Antibody CAB015417

  

Formal validation: Orthogonal

      
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

     
 

 

  

Figure description

       Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human breast shows strong cytoplasmic positivity in subset of glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 24 cell types		   RNA: detected in 37 tissues
Protein: detected in 7 cell types		   RNA: detected in 37 tissues
Protein: detected in 7 cell types		   RNA: detected in 37 tissues
Protein: detected in 15 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:700   1:800   1:800   1:600  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA006461

  

Antibody HPA056108

  

Antibody CAB005192

  

Antibody CAB015417

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

       

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		        

Target mass (kDa)

 273.4, 273.2   273.4, 273.2   273.4, 273.2, 16.7   273.4, 273.2, 16.7  

Antibody dilution

 1:250   1:100   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA006461

  

Antibody HPA056108

  

Antibody CAB005192

  

Antibody CAB015417

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:500   1:1100      

ANTIGEN INFORMATION
 
 

Antibody HPA006461

  

Antibody HPA056108

  

Antibody CAB005192

  

Antibody CAB015417

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein  

Length (aa)

 112   115      

Antigen sequence

 
RWLRVTVAGGVHISGLHTESAPRRQQEQQVPILEKFCFTPHTEEGCLSER
AALQEELQLCKGLVQALQTKVTQQGLKMVVPGLDGAQIPRDPSQQELPRL
LSAACRLQLNGN
  
MPFRGYAVLGGERGGPEVQQVPAGERPLWFICSGMGTQWRGMGLSLMRLD
RFRDSILRSDEAVKPFGLKVSQLLLSTDESTFDDIVHSFVSLTAIQIGLI
DLLSCMGLRPDGIVG
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					FASN-001 - ENSP00000304592 [100%] 
FASN-004 - ENSP00000488964 [100%] 

											 
																						FASN-001 - ENSP00000304592 [100%] 
FASN-004 - ENSP00000488964 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
FASN-001
FASN-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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