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Immunohistochemical staining of human colon shows strong cytoplasmic positivity in endothelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 12 cell types
Retrieval
HIER pH6
Antibody dilution
1:250
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA052275
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
61.5, 61.3, 61.1, 58.9, 28.6
Antibody dilution
1:210
PROTEIN ARRAY
Antibody HPA052275
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
