Fields »
ABOUT
HELP
BLOG
PWWP2A

ANTIBODY INFORMATION
 
 

Antibody HPA046733

  

Antibody HPA058233

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA046733   HPA058233  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA046733

  

Antibody HPA058233

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Uncertain    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA046733

  

Antibody HPA058233

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & focal adhesion sites.  

Antibody dilution

 1:99   1:200  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA046733

  

Antibody HPA058233

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 41 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:300    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA046733

  

Antibody HPA058233

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 82, 60.7, 56.3   82, 60.7, 56.3  

Antibody dilution

 1:490   1:850  

PROTEIN ARRAY
 
 

Antibody HPA046733

  

Antibody HPA058233

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:5000   1:10200  

ANTIGEN INFORMATION
 
 

Antibody HPA046733

  

Antibody HPA058233

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 71   71  

Antigen sequence

 
QSNTFQEGTEVKCEANGAVPDDPSPVPHPELSLAESLWTSKPPPLFHEGA
PYPPPLFIRDTYNQSIPQPPP
  
DSTVSQLIPGSEVRVTLDHIIEDALVVSFRFGEKLFSGVLMDLSKRFGPH
GIPVTVFPKREYKDKPEAMPL
 

Matching transcripts

 PWWP2A-001 - ENSP00000390462 [100%]
PWWP2A-002 - ENSP00000305151 [100%]
PWWP2A-004 - ENSP00000428143 [100%]
  PWWP2A-001 - ENSP00000390462 [100%]
PWWP2A-002 - ENSP00000305151 [100%]
PWWP2A-004 - ENSP00000428143 [100%]
 

Other gene match

    
ANTIGEN VIEW
PWWP2A-001
PWWP2A-002
PWWP2A-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.