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Antibody staining overlaps with antibody HPA054178.
Validated
Antibody staining overlaps with antibody HPA049807.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus.
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus.
Antibody dilution
1:60
1:95
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA049807
Antibody HPA054178
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human liver shows moderate cytoplasmic and membranous positivity in hepatocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 3 cell types
Retrieval
HIER pH6
Antibody dilution
1:200
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA049807
Antibody HPA054178
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
55.4
55.4
Antibody dilution
1:300
1:480
PROTEIN ARRAY
Antibody HPA049807
Antibody HPA054178
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
