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Immunohistochemical staining of human salivary gland shows strong cytoplasmic positivity in subset of glandular cells.
Immunohistochemical staining of human salivary gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human salivary gland shows strong cytoplasmic positivity in subsets of glandular cells.
Expression
RNA: detected in 9 tissues Protein: detected in 11 cell types
RNA: detected in 9 tissues Protein: detected in 5 cell types
RNA: detected in 9 tissues Protein: detected in 4 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:900
1:100
1:1000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043706
Antibody HPA044763
Antibody CAB026769
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
16.4, 16.2
16.4, 16.2
16.4
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA043706
Antibody HPA044763
Antibody CAB026769
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
