We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & cell junctions.
Immunofluorescent staining of human cell line HEK 293 shows localization to cell junctions.
Antibody dilution
1:93
1:61
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA036538
Antibody HPA060154
Standard validation
Approved
Figure description
Immunohistochemical staining of human testis shows strong membranous positivity in cells in seminiferous ducts.
Expression
RNA: detected in 34 tissues Protein: detected in 60 cell types
Retrieval
HIER pH6
Antibody dilution
1:200
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA036538
Antibody HPA060154
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407362)
Target mass (kDa)
41.6
41.6, 21.1
Antibody dilution
1:250
1:200
PROTEIN ARRAY
Antibody HPA036538
Antibody HPA060154
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
