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NPAS2

ANTIBODY INFORMATION
 
 

Antibody HPA019674

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA019674  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 6  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA019674

  
Immunocytochemistry

Supported  
Immunohistochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA019674

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

 1:50  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019674

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in cortical cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:45  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  
 

Standard validation

 Supported  

 

Figure description

 Nuclear localization in subsets of neurons including cortical pyramidal cells.  

WESTERN BLOT
 
 

Antibody HPA019674

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 91.8  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA019674

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA019674

  

Antigen

 Recombinant protein fragment  

Length (aa)

 117  

Antigen sequence

 
SKPEFIVCTHSVVSYADVRVERRQELALEDPPSEALHSSALKDKGSSLEP
RQHFNTLDVGASGLNTSHSPSASSRSSHKSSHTAMSEPTSTPTKLMAEAS
TPALPRSATLPQELPVP
 

Matching transcripts

 NPAS2-001 - ENSP00000338283 [100%]
 

Other gene match

  
ANTIGEN VIEW
NPAS2-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.