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Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.
Antibody dilution
1:123
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA039202
Antibody HPA066115
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 73 cell types
RNA: detected in 36 tissues Protein: detected in 16 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:170
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039202
Antibody HPA066115
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402086)
Target mass (kDa)
35.7, 17.1
35.7
Antibody dilution
1:250
1:88
PROTEIN ARRAY
Antibody HPA039202
Antibody HPA066115
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
