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ZNF672

ANTIBODY INFORMATION
 
 

Antibody HPA055779

  

Antibody HPA062506

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA055779   HPA062506  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA055779

  

Antibody HPA062506

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved    
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA055779

  

Antibody HPA062506

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA062506.		  
Validated

Antibody staining overlaps with antibody HPA055779.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.   Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.  

Antibody dilution

 1:33   1:39  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA055779

  

Antibody HPA062506

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 67 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:50    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA055779

  

Antibody HPA062506

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 50.2, 7.5   50.2  

Antibody dilution

 1:160   1:130  

PROTEIN ARRAY
 
 

Antibody HPA055779

  

Antibody HPA062506

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1300   1:1550  

ANTIGEN INFORMATION
 
 

Antibody HPA055779

  

Antibody HPA062506

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 54   42  

Antigen sequence

 
CYSSVLLRHERAHGGDGRFRCLECGERCARAADLRAHRRTHAGQTLYICS
ECGQ
  
SQHQRAHTRARTAAAVAIQSAVGTALVFEGPAEQEKPGFSVS
 

Matching transcripts

 ZNF672-001 - ENSP00000421915 [100%]
ZNF672-002 - ENSP00000427021 [85%]
  ZNF672-001 - ENSP00000421915 [100%]
 

Other gene match

    
ANTIGEN VIEW
ZNF672-001
ZNF672-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.