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FAM98B

ANTIBODY INFORMATION
 
 

Antibody HPA008320

  

Antibody HPA008502

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA008320   HPA008502  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   4  

References

 3    

VALIDATION SUMMARY
 
 

Antibody HPA008320

  

Antibody HPA008502

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Supported  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008320

  

Antibody HPA008502

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.  

Antibody dilution

   1:38  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008320

  

Antibody HPA008502

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human rectum shows strong membranous and nuclear positivity in glandular cells.   Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells and glial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:250  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA008320

  

Antibody HPA008502

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 45.5, 37.2   45.5, 37.2  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA008320

  

Antibody HPA008502

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA008320

  

Antibody HPA008502

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 136   126  

Antigen sequence

 
STTSDIPHMLNQVESKVKDILSKVQKNHVGKPLLKMDLNSEQAEQLERIN
DALSCEYECRRRMLMKRLDVTVQSFGWSDRAKVKTDDIARIYQPKRYALS
PKTTITMAHLLAAREDLSKIIRTSSGTSREKTACAI
  
GPQPTMEGDVLDTLEALGYKGPLLEEQALTKAAEGGLSSPEFSELCIWLG
SQIKSLCNLEESITSAGRDDLESFQLEISGFLKEMACPYSVLISGDIKDR
LKKKEDCLKLLLFLSTELQASQILQN
 

Matching transcripts

 FAM98B-001 - ENSP00000380734 [100%]
FAM98B-002 - ENSP00000453166 [100%]
  FAM98B-001 - ENSP00000380734 [100%]
FAM98B-002 - ENSP00000453166 [100%]
 

Other gene match

    
ANTIGEN VIEW
FAM98B-001
FAM98B-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA008320

  

Antibody HPA008502

  

1.

 hCLE/C14orf166 associates with DDX1-HSPC117-FAM98B in a novel transcription-dependent shuttling RNA-transporting complex
Pérez-González A et al
PLoS One 2014;9(3):e90957
    

2.

 Analysis of orthologous groups reveals archease and DDX1 as tRNA splicing factors
Popow J et al
Nature 2014;511(7507):104-7
    

3.

 The mammalian tRNA ligase complex mediates splicing of XBP1 mRNA and controls antibody secretion in plasma cells
Jurkin J et al
EMBO J 2014;33(24):2922-36
Application: WB 		   
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.