KRT13 - Antibodies - The Human Protein Atlas

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KRT13

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA030877

Antibody CAB000133

Provider

Atlas Antibodies
Sigma-Aldrich

Lab Vision/NeoMarkers

Product name

HPA030877

MS-924

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA030877

Antibody CAB000133

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA030877

Antibody CAB000133

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to intermediate filaments.

Antibody dilution

1:55

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA030877

Antibody CAB000133

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.

Immunohistochemical staining of human esophagus shows moderate cytoplasmic positivity in squamous epithelial cells.

Expression

RNA: detected in 16 tissues
Protein: detected in 15 cell types

RNA: detected in 16 tissues
Protein: detected in 5 cell types

Retrieval

HIER pH6

Antibody dilution

1:2500

1:100

Literature conformity

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA030877

Antibody CAB000133

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

49.6, 45.9, 45.3, 16

49.6, 45.9, 45.3, 19.2, 16

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA030877

Antibody CAB000133

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA030877

Antibody CAB000133

Antigen

Recombinant protein fragment

Not known

Length (aa)

Antigen sequence

MSLRLQSSSASYGGGFGGGSCQLGGGRGVSTCSTRFVSGGSAGGYGGGVS
CG

Matching transcripts

KRT13-001 - ENSP00000246635 [100%]
KRT13-002 - ENSP00000336604 [100%]
KRT13-005 - ENSP00000468221 [100%]
KRT13-008 - ENSP00000467833 [100%]

Other gene match

ANTIGEN VIEW

KRT13-001	KRT13-002	KRT13-005	KRT13-008

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.