MCC - Antibodies - The Human Protein Atlas

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MCC

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA037390

Antibody HPA037391

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA037390

HPA037391

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA037390

Antibody HPA037391

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA037390

Antibody HPA037391

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Antibody dilution

1:122

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA037390

Antibody HPA037391

Standard validation

Approved

Figure description

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferus ducts.

Immunohistochemical staining of human ovary shows moderate cytoplasmic positivity in ovarian stroma cells.

Expression

RNA: detected in 35 tissues
Protein: detected in 32 cell types

RNA: detected in 35 tissues
Protein: detected in 75 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:500

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

Standard validation

Approved

Figure description

Neuron-like and astrocyte-like staining throughout the brain. JM staining intensity is a bit uneven.

WESTERN BLOT

Antibody HPA037390

Antibody HPA037391

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

113.3, 93, 86.1

113.3, 93, 79.9

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA037390

Antibody HPA037391

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA037390

Antibody HPA037391

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

VQELVSALERLTKSSEIRHQQSAEFVNDLKRANSNLVAAYEKAKKKHQNK
LKKLESQMMAMVERHETQVRMLKQRIALLEEENSRPHTNETS

ALASLKGDIVELNKRLQQTERERDLLEKKLAKAQCEQSHLMREHEDVQER
TTLRYEERITELHSVIAELNKKIDRLQGTTIREEDEYSELRSEL

Matching transcripts

MCC-001 - ENSP00000305617 [100%]
MCC-003 - ENSP00000386227 [100%]
MCC-011 - ENSP00000421615 [100%]

MCC-001 - ENSP00000305617 [100%]
MCC-003 - ENSP00000386227 [100%]
MCC-009 - ENSP00000485220 [100%]

Other gene match

ANTIGEN VIEW

MCC-001	MCC-003	MCC-009	MCC-011

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.