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Gene name

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Reliability

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Method

Validation

Method

Validation

Method

Validation

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Expression

Tissue

Specificity

Cell line

Specificity

Tissue

Detectable

Cell line

Detectable

Category

Score

Score

Score

Antibodies

Column

 
 
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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA027529

 

Antibody CAB033120

 

Provider

Atlas Antibodies
Sigma-Aldrich
  Santa Cruz Biotechnology
 

Product name

HPA027529   sc-33582  

Host species

Rabbit   Rabbit  

Clonality

pAb   pAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

   

Released in version

6   14  

References

1    

VALIDATION SUMMARY

 
 

Antibody HPA027529

 

Antibody CAB033120

 
Immunohistochemistry

Supported  

Validated
 
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY

 
 

Antibody HPA027529

 

Antibody CAB033120

 

Formal validation: Orthogonal

 
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.
 

 
 

 

 

Figure description

  Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

Supported   Supported  

   

Figure description

Immunohistochemical staining of human gall bladder shows distinct positivity in plasma.   Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells. Extracellular material/ plasma distinctly stained.  

Expression

RNA: detected in 9 tissues
Protein: detected in 7 cell types
  RNA: detected in 9 tissues
Protein: detected in 16 cell types
 

Retrieval

HIER pH6   HIER pH6  

Antibody dilution

1:150   1:300  

Literature conformity

Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT

 
 

Antibody HPA027529

 

Antibody CAB033120

 

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.
  Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.
 

   

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
 

Target mass (kDa)

52.3, 51.5, 50.3, 49.5, 14, 13.5   52.3, 51.5, 50.3, 49.5, 14, 13.5  

Antibody dilution

1:250   1:500  

PROTEIN ARRAY

 
 

Antibody HPA027529

 

Antibody CAB033120

 

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.
   

   

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

1:500    

ANTIGEN INFORMATION

 
 

Antibody HPA027529

 

Antibody CAB033120

 

Antigen

Recombinant protein fragment   Recombinant protein  

Length (aa)

149    

Antigen sequence

MIDAATLKSRKMLEEIMKYEASILTHDSSIRYLQEIYNSNNQKIVNLKEK
VAQLEAQCQEPCKDTVQIHDITGKDCQDIANKGAKQSGLYFIKPLKANQQ
FLVYCEIDGSGNGWTVFQKRLDGSVDFKKNWIQYKEGFGHLSPTGTTEF
   

Matching transcripts

FGG-001 - ENSP00000384860 [100%]
FGG-002 - ENSP00000336829 [100%]
FGG-006 - ENSP00000384101 [95%]
FGG-007 - ENSP00000384552 [95%]
FGG-008 - ENSP00000407562 [83%]
FGG-009 - ENSP00000377429 [80%]
   

Other gene match

   
ANTIGEN VIEW
FGG-001
FGG-002
FGG-006
FGG-007
FGG-008
FGG-009

RELEVANT PUBLICATIONS

 
 

Antibody HPA027529

 

Antibody CAB033120

 

1.

The human liver-specific proteome defined by transcriptomics and antibody-based profiling
Kampf C et al
FASEB J 2014;28(7):2901-14
   
 

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