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GPHN

ANTIBODY INFORMATION
 
 

Antibody HPA003116

  

Antibody HPA024694

  

Antibody CAB004419

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA003116   HPA024694   sc-25311  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 2   5   3  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA003116

  

Antibody HPA024694

  

Antibody CAB004419

  
Immunocytochemistry

  Uncertain    
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003116

  

Antibody HPA024694

  

Antibody CAB004419

  

Standard validation

   Uncertain    

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to cytoplasmic bodies.    

Antibody dilution

   1:56    

Literature conformity

   The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003116

  

Antibody HPA024694

  

Antibody CAB004419

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  
 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA024694 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA003116 across 46 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human lateral ventricle shows moderate cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 26 cell types		   RNA: detected in 37 tissues
Protein: detected in 35 cell types		   RNA: detected in 37 tissues
Protein: detected in 69 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:15   1:200   1:3000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003116

  

Antibody HPA024694

  

Antibody CAB004419

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 84.7, 83.4, 79.7, 21.7   84.7, 83.4, 79.7   84.7, 83.4, 79.7, 41.4, 30.1, 21.7, 6.8  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA003116

  

Antibody HPA024694

  

Antibody CAB004419

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA003116

  

Antibody HPA024694

  

Antibody CAB004419

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 142   140    

Antigen sequence

 
ATIQEHGYPTINLGIVGDNPDDLLNALNEGISRADVIITSGGVSMGEKDY
LKQVLDIDLHAQIHFGRVFMKPGLPTTFATLDIDGVRKIIFALPGNPVSA
VVTCNLFVVPALRKMQGILDPRPTIIKARLSCDVKLDPRPEY
  
SRCSSKENILRASHSAVDITKVARRHRMSPFPLTSMDKAFITVLEMTPVL
GTEIINYRDGMGRVLAQDVYAKDNLPPFPASVKDGYAVRAADGPGDRFII
GESQAGEQPTQTVMPGQVMRVTTGAPIPCGADAVVQVEDT
  

											 
																				

																		

										
											
Matching transcripts

												
																					GPHN-003 - ENSP00000312771 [100%] 
GPHN-006 - ENSP00000417901 [100%] 
GPHN-007 - ENSP00000438404 [100%] 
GPHN-017 - ENSP00000452009 [93%] 

											 
																						GPHN-003 - ENSP00000312771 [100%] 
GPHN-006 - ENSP00000417901 [100%] 
GPHN-007 - ENSP00000438404 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
GPHN-003
GPHN-006
GPHN-007
GPHN-017
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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