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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.
Antibody dilution
1:21
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA042754
Antibody HPA043398
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human lateral ventricle shows distinct cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Expression
RNA: detected in 37 tissues Protein: detected in 18 cell types
RNA: detected in 37 tissues Protein: detected in 8 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA042754
Antibody HPA043398
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY421589)
Target mass (kDa)
27.7, 27.3, 23.6
27.7, 27.3, 23.6
Antibody dilution
1:125
1:125
PROTEIN ARRAY
Antibody HPA042754
Antibody HPA043398
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
