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Antibody staining overlaps with antibody HPA063358.
Validated
Antibody staining overlaps with antibody HPA060247.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.
Antibody dilution
1:55
1:167
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA060247
Antibody HPA063358
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human esophagus shows moderate nuclear positivity in squamous epithelial cells.
Expression
RNA: detected in 33 tissues Protein: detected in 43 cell types
Retrieval
HIER pH6
Antibody dilution
1:75
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA060247
Antibody HPA063358
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
60.6, 19.9
60.6
Antibody dilution
1:180
1:550
PROTEIN ARRAY
Antibody HPA060247
Antibody HPA063358
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
