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LAMB2

ANTIBODY INFORMATION
 
 

Antibody HPA001895

  

Antibody HPA050033

  

Antibody CAB000053

  

Antibody CAB078160

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001895   HPA050033   Z0097   AMAb91097  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

        

Released in version

 2   12   1   16  

References

 1        

VALIDATION SUMMARY
 
 

Antibody HPA001895

  

Antibody HPA050033

  

Antibody CAB000053

  

Antibody CAB078160

  
Immunocytochemistry

  Uncertain      
Immunohistochemistry


Validated
     Supported   Supported  
Western blot

Supported   Uncertain   Supported    
Protein array

Approved   Approved      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001895

  

Antibody HPA050033

  

Antibody CAB000053

  

Antibody CAB078160

  

Standard validation

   Uncertain      

       

Figure description

   Immunofluorescent staining of human cell line HeLa shows localization to vesicles.      

Antibody dilution

   1:64      

Literature conformity

   The subcellular location is not consistent with literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001895

  

Antibody HPA050033

  

Antibody CAB000053

  

Antibody CAB078160

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		        

 

 

        

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.        
 

Standard validation

 Supported     Supported   Supported  

       

Figure description

 Immunohistochemical staining of human heart muscle shows distinct membranous positivity in myocytes.     Immunohistochemical staining of human heart muscle shows strong cytoplasmic and membranous positivity in myocytes.   Immunohistochemical staining of human placenta shows strong positivity in endothelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 59 cell types		     RNA: detected in 37 tissues
Protein: detected in 22 cell types		   RNA: detected in 37 tissues
Protein: detected in 8 cell types		  

Retrieval

 HIER pH6     Proteinase K.   HIER pH6  

Antibody dilution

 1:100     1:500   1:8000  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.     Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001895

  

Antibody HPA050033

  

Antibody CAB000053

  

Antibody CAB078160

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		    

       

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 196   196   196, 17.3    

Antibody dilution

 1:250   1:320   1:500    

PROTEIN ARRAY
 
 

Antibody HPA001895

  

Antibody HPA050033

  

Antibody CAB000053

  

Antibody CAB078160

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:2550      

ANTIGEN INFORMATION
 
 

Antibody HPA001895

  

Antibody HPA050033

  

Antibody CAB000053

  

Antibody CAB078160

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Native protein   Recombinant protein  

Length (aa)

 133   129      

Antigen sequence

 
DLTDVQDENFNANHALSGLERDRLALNLTLRQLDQHLDLLKHSNFLGAYD
SIRHAHSQSAEAERRANTSALAVPSPVSNSASARHRTEALMDAQKEDFNS
KHMANQRALGKLSAHTHTLSLTDINELVCGAPG
  
SLCGHLVPKDDRIQGTLQPHARYLIFPNPVCLEPGISYKLHLKLVRTGGS
AQPETPYSGPGLLIDSLVLLPRVLVLEMFSGGDAAALERQATFERYQCHE
EGLVPSKTSPSEACAPLLISLSTLIYNGA
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					LAMB2-001 - ENSP00000388325 [100%] 
LAMB2-003 - ENSP00000307156 [100%] 

											 
																						LAMB2-001 - ENSP00000388325 [100%] 
LAMB2-003 - ENSP00000307156 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
LAMB2-001
LAMB2-003
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001895

												
										 
																				
											
											
											
Antibody HPA050033

												
										 
																				
											
											
											
Antibody CAB000053

												
										 
																				
											
											
											
Antibody CAB078160

												
										 
																		

																	

										
											
1.

												
																						A recellularized human colon model identifies cancer driver genes

	Chen HJ et al

	Nat Biotechnol 2016;34(8):845-51

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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