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ANTIBODY INFORMATION
 
 

Antibody HPA036986

  

Antibody HPA036987

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036986   HPA036987  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA036986

  

Antibody HPA036987

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036986

  

Antibody HPA036987

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA036987.		  
Validated

Antibody staining overlaps with antibody HPA036986.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.   Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.  

Antibody dilution

 1:127   1:60  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036986

  

Antibody HPA036987

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA036987 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA036986 across 46 cell lines.		  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA036987 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA036986 across 74 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 59 cell types		   RNA: detected in 37 tissues
Protein: detected in 63 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:100  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036986

  

Antibody HPA036987

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 87.8, 86.6, 71.7, 28.6   87.8, 86.6, 38.5, 21.4, 20.8, 20.4, 19.1  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA036986

  

Antibody HPA036987

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA036986

  

Antibody HPA036987

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 86   101  

Antigen sequence

 
HTGYVIELQHVVKGPSGCVESLEVTCRRADAGEKPKAFIHWVSQPLMCEV
RLYERLFQHKNPEDPTEVPGGFLSDLNLASLHVVDA
  
LSFLVSYIASKKIHTEPQLSAALEYVRSHPLDPIDTVDFERECGVGVIVT
PEQIEEAVEAAINRHRPQLLVERYHFNMGLLMGEARAVLKWADGKMIKNE
V
 

Matching transcripts

 QARS-001 - ENSP00000307567 [100%]
QARS-005 - ENSP00000390015 [100%]
QARS-006 - ENSP00000489011 [100%]
QARS-009 - ENSP00000396326 [91%]
  QARS-001 - ENSP00000307567 [100%]
QARS-033 - ENSP00000489550 [100%]
QARS-037 - ENSP00000488960 [100%]
QARS-047 - ENSP00000489154 [100%]
QARS-039 - ENSP00000489082 [93%]
QARS-005 - ENSP00000390015 [88%]
QARS-042 - ENSP00000489056 [84%]
 

Other gene match

    
ANTIGEN VIEW
QARS-001
QARS-005
QARS-006
QARS-009
QARS-033
QARS-037
QARS-039
QARS-042
QARS-047
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.