We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Antibody dilution
1:125
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA042416
Antibody HPA062017
Antibody CAB023353
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human pituitary gland shows strong membranous and cytoplasmic positivity in anterior lobe.
Immunohistochemical staining of human pituitary gland shows strong membranous and cytoplasmic positivity in anterior lobe.
Immunohistochemical staining of human pituitary gland shows strong cytoplasmic and membranous positivity in anterior lobe.
Expression
RNA: detected in 5 tissues Protein: detected in 2 cell types
RNA: detected in 5 tissues Protein: detected in 6 cell types
RNA: detected in 5 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:4000
1:4500
1:6000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA042416
Antibody HPA062017
Antibody CAB023353
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
25.9, 22.6
25.9
25.9, 22.6
Antibody dilution
1:250
1:610
1:500
PROTEIN ARRAY
Antibody HPA042416
Antibody HPA062017
Antibody CAB023353
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
