GNB2 - Antibodies - The Human Protein Atlas

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GNB2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA040736

Antibody CAB010032

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA040736

sc-380

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

GNB1 - ENSG00000078369 [90%]
GNB3 - ENSG00000111664 [83%]
GNB4 - ENSG00000114450 [100%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA040736

Antibody CAB010032

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA040736

Antibody CAB010032

Standard validation

Approved

Uncertain

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

Antibody dilution

1:9

1:100

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

The subcellular location is not consistent with literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA040736

Antibody CAB010032

Standard validation

Approved

Figure description

Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.

Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 48 cell types

Retrieval

HIER pH6

Antibody dilution

1:200

1:1200

Literature conformity

Consistent with gene/protein characterization data.

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA040736

Antibody CAB010032

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

37.6, 37.4, 37.3, 37.2, 37.1, 32.4, 29.3, 25.9

37.3, 32.4, 27.6, 25.9, 25.5, 17

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA040736

Antibody CAB010032

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

ANTIGEN INFORMATION

Antibody HPA040736

Antibody CAB010032

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

Antigen sequence

CRLFDLRADQELLLYSHDNIICGITSVAF

Matching transcripts

GNB2-001 - ENSP00000305260 [97%]
GNB2-002 - ENSP00000401873 [97%]
GNB2-003 - ENSP00000389391 [97%]
GNB2-004 - ENSP00000390543 [97%]
GNB2-005 - ENSP00000400286 [97%]
GNB2-006 - ENSP00000377503 [97%]
GNB2-007 - ENSP00000377501 [97%]

Other gene match

GNB1 - ENSG00000078369 [90%]
GNB3 - ENSG00000111664 [83%]
GNB4 - ENSG00000114450 [100%]

ANTIGEN VIEW

GNB2-001	GNB2-002	GNB2-003	GNB2-004	GNB2-005	GNB2-006	GNB2-007

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.