SYNPO2 - Antibodies - The Human Protein Atlas

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SYNPO2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA030665

Antibody HPA049707

Antibody HPA068563

Antibody CAB037231

Provider

Atlas Antibodies
Sigma-Aldrich

SIGMA

Product name

HPA030665

HPA049707

HPA068563

M9818

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA030665

Antibody HPA049707

Antibody HPA068563

Antibody CAB037231

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA030665

Antibody HPA049707

Antibody HPA068563

Antibody CAB037231

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA068563.

Validated

Antibody staining overlaps with antibody HPA049707.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol, actin filaments & vesicles.

Antibody dilution

1:152

1:200

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA030665

Antibody HPA049707

Antibody HPA068563

Antibody CAB037231

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA068563 across 74 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA030665 across 74 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.

Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Expression

RNA: detected in 35 tissues
Protein: detected in 22 cell types

RNA: detected in 35 tissues
Protein: detected in 10 cell types

RNA: detected in 35 tissues
Protein: detected in 57 cell types

Retrieval

HIER pH6

Antibody dilution

1:1000

1:5000

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA030665

Antibody HPA049707

Antibody HPA068563

Antibody CAB037231

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

136.3, 133.1, 125.3, 119.2, 117.5

136.3, 133.1, 125.3, 119.2, 117.5, 13.4

Antibody dilution

1:250

1:760

1:1420

1:500

PROTEIN ARRAY

Antibody HPA030665

Antibody HPA049707

Antibody HPA068563

Antibody CAB037231

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:30000

1:6100

1:17050

ANTIGEN INFORMATION

Antibody HPA030665

Antibody HPA049707

Antibody HPA068563

Antibody CAB037231

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

Antigen sequence

EDKVGGTPSREQDAAQTDGLRTTTSYQRKEEESVRTQSSVSKSYIEVSHG
LGHVPQQNGFSGASETANIQRMVPMNRTAKPFPGSVNQPAT

YPEVIKLMESITDSLQMLIKRPSSGISEALISENENKNLEHLTHGGYVES
TTLQIRPATKTQCTEFFLAPVKTEVPLAENQRSGPD

DPNLSHDRIVHINSIPTNEKADPFLRSSKIIQISSGRELRVIQESEAGDA
GLPRVEVILDCSDRQKTEGCRLQAGKECVDSPVEG

Matching transcripts

SYNPO2-001 - ENSP00000306015 [99%]
SYNPO2-002 - ENSP00000390965 [99%]
SYNPO2-003 - ENSP00000395143 [99%]
SYNPO2-004 - ENSP00000425496 [99%]
SYNPO2-006 - ENSP00000484885 [99%]

SYNPO2-001 - ENSP00000306015 [100%]
SYNPO2-002 - ENSP00000390965 [100%]
SYNPO2-003 - ENSP00000395143 [100%]
SYNPO2-004 - ENSP00000425496 [100%]
SYNPO2-006 - ENSP00000484885 [100%]

Other gene match

ANTIGEN VIEW

SYNPO2-001	SYNPO2-002	SYNPO2-003	SYNPO2-004	SYNPO2-006

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.