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ZNF621

ANTIBODY INFORMATION
 
 

Antibody HPA065518

  

Antibody HPA071440

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA065518   HPA071440  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA065518

  

Antibody HPA071440

  
Immunocytochemistry


Validated
  

Validated
  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA065518

  

Antibody HPA071440

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA071440.		  
Validated

Antibody staining overlaps with antibody HPA065518.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.   Immunofluorescent staining of human cell line HeLa shows localization to nuclear speckles.  

Antibody dilution

 1:44   1:30  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

WESTERN BLOT
 
 

Antibody HPA065518

  

Antibody HPA071440

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 49.2, 17.7, 17.3   49.2, 36.7  

Antibody dilution

 1:150   1:80  

PROTEIN ARRAY
 
 

Antibody HPA065518

  

Antibody HPA071440

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1750   1:1100  

ANTIGEN INFORMATION
 
 

Antibody HPA065518

  

Antibody HPA071440

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 61   93  

Antigen sequence

 
RGISQGGESWIKNEGLVIKQEASEETELHRMPVGGLLRNVSQHFDFKRKA
LKQTFNLNPNL
  
WYGSFVQHQKLHPVEKKPVKVLGPSLVSPQCSSPAIPPVLLQGSCSASAV
AVPSLTFPHAVLIPTSGNFFMLLPTSGIPSSSAQIVRVFQGLT
 

Matching transcripts

 ZNF621-001 - ENSP00000340841 [100%]
ZNF621-002 - ENSP00000386051 [100%]
ZNF621-005 - ENSP00000408779 [100%]
ZNF621-201 - ENSP00000312144 [100%]
  ZNF621-001 - ENSP00000340841 [100%]
ZNF621-002 - ENSP00000386051 [100%]
ZNF621-003 - ENSP00000413236 [100%]
 

Other gene match

    
ANTIGEN VIEW
ZNF621-001
ZNF621-002
ZNF621-003
ZNF621-005
ZNF621-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.