NBEA - Antibodies - The Human Protein Atlas

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NBEA

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA039730

Antibody HPA040385

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA039730

HPA040385

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA039730

Antibody HPA040385

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA039730

Antibody HPA040385

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus, nucleoli & the Golgi apparatus.

Antibody dilution

1:88

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA039730

Antibody HPA040385

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA040385 across 71 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA039730 across 71 cell types.

Standard validation

Approved

Figure description

Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.

Expression

RNA: detected in 32 tissues
Protein: detected in 52 cell types

RNA: detected in 32 tissues
Protein: detected in 50 cell types

Retrieval

HIER pH6

Antibody dilution

1:600

1:85

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA039730

Antibody HPA040385

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.

Figure description

Target mass (kDa)

328, 327.9, 327.8, 327.5

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA039730

Antibody HPA040385

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:6000

ANTIGEN INFORMATION

Antibody HPA039730

Antibody HPA040385

Antigen

Recombinant protein fragment

Length (aa)

116

101

Antigen sequence

ETTRTGSQPGRNIRQEINSPTSTVVVIPSIPHPSLNHGFLAKLIPEQSFG
HSFYKETPAAFPDTIKEKETPTPGEDIQVESSIPHTDSGIGEEQVASILN
GAELETSTGPDAMSEL

FKIHTTSDGMSSISERDLASSTKGLEYAEMTATTLETESSSSKIVPNIDA
GSIISDTERSDDGKESGKEIRKIQTTTTTQAVQGRSITQQDRDLRVDLGF
R

Matching transcripts

NBEA-001 - ENSP00000369271 [100%]
NBEA-201 - ENSP00000308534 [100%]
NBEA-202 - ENSP00000383295 [100%]
NBEA-204 - ENSP00000486239 [100%]

Other gene match

ANTIGEN VIEW

NBEA-001	NBEA-201	NBEA-202	NBEA-204

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.