Fields »
ABOUT
HELP
BLOG
KDM2A

ANTIBODY INFORMATION
 
 

Antibody HPA044251

  

Antibody CAB012272

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Abcam plc
  

Product name

 HPA044251   ab 31739  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 12   4  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA044251

  

Antibody CAB012272

  
Immunocytochemistry

Uncertain    
Immunohistochemistry

  Supported  
Western blot

Supported   Uncertain  
Protein array

Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA044251

  

Antibody CAB012272

  

Standard validation

 Uncertain    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.    

Antibody dilution

 1:200    

Literature conformity

 The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA044251

  

Antibody CAB012272

  

Standard validation

   Supported  

   

Figure description

   Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 76 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:75  

Literature conformity

   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA044251

  

Antibody CAB012272

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 132.8, 130.7, 89.9, 81.7   132.8, 130.7, 89.9, 81.7  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA044251

  

Antibody CAB012272

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA044251

  

Antibody CAB012272

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 85    

Antigen sequence

 
IEDEDALIADVKILLEELANSDPKLALTGVPIVQWPKRDKLKFPTRPKVR
VPTIPITKPHTMKPAPRLTPVRPAAASPIVSGARR
  

											 
																				

																		

										
											
Matching transcripts

												
																					KDM2A-001 - ENSP00000381640 [100%] 
KDM2A-002 - ENSP00000432786 [100%] 
KDM2A-201 - ENSP00000309302 [100%] 
KDM2A-010 - ENSP00000435776 [87%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
KDM2A-001
KDM2A-002
KDM2A-010
KDM2A-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.