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Antibody staining overlaps with antibody HPA077682.
Validated
Antibody staining overlaps with antibody HPA017730.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments.
Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments.
Antibody dilution
1:4
1:26
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA017730
Antibody HPA077682
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human urinary bladder shows strong membranous and cytoplasmic positivity in urothelial cells.
Expression
RNA: detected in 33 tissues Protein: detected in 37 cell types
Retrieval
HIER pH6
Antibody dilution
1:20
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA017730
Antibody HPA077682
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
138.9, 103.9, 102.6, 55.5
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA017730
Antibody HPA077682
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
