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TOPAZ1

ANTIBODY INFORMATION
 
 

Antibody HPA047431

  

Antibody HPA051034

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA047431   HPA051034  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA047431

  

Antibody HPA051034

  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047431

  

Antibody HPA051034

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human stomach, upper shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 68 cell types		   RNA: detected in 1 tissues
Protein: detected in 50 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:250  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA047431

  

Antibody HPA051034

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 190.9   190.9  

Antibody dilution

 1:240   1:270  

PROTEIN ARRAY
 
 

Antibody HPA047431

  

Antibody HPA051034

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1900   1:2150  

ANTIGEN INFORMATION
 
 

Antibody HPA047431

  

Antibody HPA051034

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 102   95  

Antigen sequence

 
RSASEELKLSCQRTIPMTGKRTWPYYSCARISAWCWKKASLPESSYFLRG
SQESCRQVDVPKHQTNQTHLTDSKLLLQSSLTETNTESSSKEKLDSNSNC
LS
  
KSDTNSIPQLLQTEENVMGVNKLLPEESDLYQSKTNGLLSCLQHEKNKYS
IEESSVGRKPRKRMKLSEKADETVTEMNFSNEYNKSELMLQENQM
 

Matching transcripts

 TOPAZ1-001 - ENSP00000310303 [100%]
  TOPAZ1-001 - ENSP00000310303 [100%]
 

Other gene match

    
ANTIGEN VIEW
TOPAZ1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.