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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Antibody dilution
1:23
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA036452
Antibody HPA058138
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human skeletal muscle shows distinct nuclear and cytoplasmic positivity in myocytes.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.
Expression
RNA: detected in 21 tissues Protein: detected in 61 cell types
RNA: detected in 21 tissues Protein: detected in 58 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:20
1:250
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA036452
Antibody HPA058138
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY422985)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
42.6, 38.7, 11.3
42.6
Antibody dilution
1:250
1:320
PROTEIN ARRAY
Antibody HPA036452
Antibody HPA058138
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
