Cell atlas - PRPF8 - The Human Protein Atlas

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PRPF8

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TISSUE

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CELL

CANCER

CELL ATLAS RNA EXPRESSION HUMAN CELLS

GENE/PROTEIN

Antibody validation

Dictionary
	Nucleoplasm

Human cell
	Nucleoplasm

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GENERAL INFORMATION
Gene name	PRPF8
Gene description	Pre-mRNA processing factor 8
Protein class	Cancer-related genes Disease related genes Plasma proteins Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	5
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Localized to the nucleoplasm.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nucleoplasm (validated)

DATA RELIABILITY
Reliability score	Validated based on 2 antibodies. CAB009941 , CAB015457
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ASSAYS

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Localized to the nucleoplasm.

Main location

Nucleoplasm (validated)


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail	Antibody	Cell line	Location	Single-cell variation	Cell cycle dependent variation
	CAB015457	A-431	No staining
	CAB015457	U-2 OS	Nucleoplasm
	CAB015457	U-251 MG	Nucleoplasm
	CAB009941	A-431	Nucleoplasm
	CAB009941	U-2 OS	Nucleoplasm
	CAB009941	U-251 MG	Nucleoplasm

GENE INFORMATION
Gene name	PRPF8 (HGNC Symbol)
Synonyms	hPrp8, Prp8, PRPC8, RP13, SNRNP220
Description	Pre-mRNA processing factor 8 (HGNC Symbol)
Entrez gene summary	Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008]
Chromosome	17
Cytoband	p13.3
Chromosome location (bp)	1650629 - 1684882
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000174231 (version 83.38)
Entrez gene	10594
UniProt	Q6P2Q9 (UniProt - Evidence at protein level)
neXtProt	NX_Q6P2Q9
Antibodypedia	PRPF8 antibodies

PROTEIN BROWSER

PRPF8-001	PRPF8-002	PRPF8-003	PRPF8-013	PRPF8-017

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
PRPF8-001 ENSP00000304350 ENST00000304992	Q6P2Q9 [Direct mapping] Pre-mRNA-processing-splicing factor 8 Show all	SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins Predicted intracellular proteins Plasma proteins Cancer-related genes Mutational cancer driver genes Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000244 [spliceosomal tri-snRNP complex assembly] GO:0000375 [RNA splicing, via transesterification reactions] GO:0000386 [second spliceosomal transesterification activity] GO:0000398 [mRNA splicing, via spliceosome] GO:0003676 [nucleic acid binding] GO:0003723 [RNA binding] GO:0005515 [protein binding] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005682 [U5 snRNP] GO:0006397 [mRNA processing] GO:0008380 [RNA splicing] GO:0010467 [gene expression] GO:0016020 [membrane] GO:0016607 [nuclear speck] GO:0017070 [U6 snRNA binding] GO:0030619 [U1 snRNA binding] GO:0030620 [U2 snRNA binding] GO:0030623 [U5 snRNA binding] GO:0044822 [poly(A) RNA binding] GO:0070530 [K63-linked polyubiquitin binding] GO:0071013 [catalytic step 2 spliceosome] GO:0097157 [pre-mRNA intronic binding] Show all	2335 aa 273.6 kDa	No	0
PRPF8-002 ENSP00000460348 ENST00000572621	Q6P2Q9 [Direct mapping] Pre-mRNA-processing-splicing factor 8 Show all	SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins Predicted intracellular proteins Plasma proteins Cancer-related genes Mutational cancer driver genes Disease related genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0000244 [spliceosomal tri-snRNP complex assembly] GO:0000375 [RNA splicing, via transesterification reactions] GO:0000386 [second spliceosomal transesterification activity] GO:0000398 [mRNA splicing, via spliceosome] GO:0003676 [nucleic acid binding] GO:0003723 [RNA binding] GO:0005515 [protein binding] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005682 [U5 snRNP] GO:0006397 [mRNA processing] GO:0008380 [RNA splicing] GO:0010467 [gene expression] GO:0016020 [membrane] GO:0016607 [nuclear speck] GO:0017070 [U6 snRNA binding] GO:0030532 [small nuclear ribonucleoprotein complex] GO:0030619 [U1 snRNA binding] GO:0030620 [U2 snRNA binding] GO:0030623 [U5 snRNA binding] GO:0044822 [poly(A) RNA binding] GO:0070530 [K63-linked polyubiquitin binding] GO:0071013 [catalytic step 2 spliceosome] GO:0071222 [cellular response to lipopolysaccharide] GO:0071356 [cellular response to tumor necrosis factor] GO:0097157 [pre-mRNA intronic binding] Show all	2335 aa 273.6 kDa	No	0
PRPF8-003 ENSP00000458151 ENST00000577001	I3L0J9 [Direct mapping] Pre-mRNA-processing-splicing factor 8 Show all	MEMSAT3 predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0000386 [second spliceosomal transesterification activity] GO:0000398 [mRNA splicing, via spliceosome] GO:0003676 [nucleic acid binding] GO:0003723 [RNA binding] GO:0005681 [spliceosomal complex] GO:0017070 [U6 snRNA binding] GO:0030619 [U1 snRNA binding] GO:0030620 [U2 snRNA binding] GO:0030623 [U5 snRNA binding] GO:0097157 [pre-mRNA intronic binding] Show all	1013 aa 119.8 kDa	No	0
PRPF8-013 ENSP00000460849 ENST00000573725	I3L3Z8 [Direct mapping] Pre-mRNA-processing-splicing factor 8 Show all	Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0000375 [RNA splicing, via transesterification reactions] GO:0000386 [second spliceosomal transesterification activity] GO:0017070 [U6 snRNA binding] GO:0030619 [U1 snRNA binding] GO:0030620 [U2 snRNA binding] GO:0030623 [U5 snRNA binding] GO:0097157 [pre-mRNA intronic binding] Show all	250 aa 29.4 kDa	No	0
PRPF8-017 ENSP00000459095 ENST00000571958	I3L1T8 [Direct mapping] Pre-mRNA-processing-splicing factor 8 Show all	SPOCTOPUS predicted secreted proteins Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0000375 [RNA splicing, via transesterification reactions] GO:0000386 [second spliceosomal transesterification activity] GO:0017070 [U6 snRNA binding] GO:0030619 [U1 snRNA binding] GO:0030620 [U2 snRNA binding] GO:0030623 [U5 snRNA binding] GO:0097157 [pre-mRNA intronic binding] Show all	116 aa 12.4 kDa	No	0

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.