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SLC6A19

ANTIBODY INFORMATION
 
 

Antibody HPA037415

  

Antibody HPA043207

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA037415   HPA043207  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA037415

  

Antibody HPA043207

  
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037415

  

Antibody HPA043207

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA043207 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA037415 across 74 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.  

Expression

 RNA: detected in 7 tissues
Protein: detected in 8 cell types		   RNA: detected in 7 tissues
Protein: detected in 7 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:5000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA037415

  

Antibody HPA043207

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 71.1   71.1  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA037415

  

Antibody HPA043207

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:30000  

ANTIGEN INFORMATION
 
 

Antibody HPA037415

  

Antibody HPA043207

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 71   38  

Antigen sequence

 
GFRATQRYDDCFSTNILTLINGFDLPEGNVTQENFVDMQQRCNASDPAAY
AQLVFQTCDINAFLSEAVEGT
  
MVRLVLPNPGLDARIPSLAELETIEQEEASSRPKWDNK
 

Matching transcripts

 SLC6A19-001 - ENSP00000305302 [100%]
  SLC6A19-001 - ENSP00000305302 [100%]
 

Other gene match

    
ANTIGEN VIEW
SLC6A19-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.