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KIAA1551

ANTIBODY INFORMATION
 
 

Antibody HPA013816

  

Antibody HPA015622

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA013816   HPA015622  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   4  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA013816

  

Antibody HPA015622

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA013816

  

Antibody HPA015622

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & intermediate filaments.  

Antibody dilution

 1:38   1:133  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA013816

  

Antibody HPA015622

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.   Immunohistochemical staining of human cerebellum shows cytoplasmic and nuclear positivity in Purkinje cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 69 cell types		   RNA: detected in 37 tissues
Protein: detected in 68 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:135  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA013816

  

Antibody HPA015622

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 194.9   194.9  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA013816

  

Antibody HPA015622

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA013816

  

Antibody HPA015622

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 133   138  

Antigen sequence

 
LTKLESSPRKLHKDKRQENKHKTFLPVKGNTEKSNMLEFKLCPDILLKNT
NSVEERKDVKPHPRKEQAPLQVSGIKSTKEDWLKFVATKKRTQKDSQERD
NVNSRLSKRSFSADGFEMLQNPVKDSKEMFQTY
  
KEALSNKASKKICVKNVPCDSEHMRPSKLAVQVESCGKSNEKHSSGVQTS
KESLNGLTSHGKNLKIHHSQESKTYNILRNVKEKVGGKQPDKIWIDKTKL
DKLTNISNEAQFSQMPPQVKDQKKLYLNRVGFKCTERE
 

Matching transcripts

 KIAA1551-001 - ENSP00000310338 [100%]
  KIAA1551-001 - ENSP00000310338 [100%]
 

Other gene match

    
ANTIGEN VIEW
KIAA1551-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.