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PCCA

ANTIBODY INFORMATION
 
 

Antibody HPA041716

  

Antibody HPA047792

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041716   HPA047792  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   10  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA041716

  

Antibody HPA047792

  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Uncertain   Supported  
Protein array

Approved   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041716

  

Antibody HPA047792

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA047792 across 72 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA041716 across 72 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 61 cell types		   RNA: detected in 37 tissues
Protein: detected in 32 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041716

  

Antibody HPA047792

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 80.1, 77   80.1, 77, 75  

Antibody dilution

 1:250   1:80  

PROTEIN ARRAY
 
 

Antibody HPA041716

  

Antibody HPA047792

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA041716

  

Antibody HPA047792

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 106   94  

Antigen sequence

 
GSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKV
NILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQE
ICVIEA
  
YPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGV
RVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNY
 

Matching transcripts

 PCCA-001 - ENSP00000365462 [100%]
PCCA-202 - ENSP00000365463 [100%]
  PCCA-001 - ENSP00000365462 [100%]
PCCA-201 - ENSP00000365456 [100%]
PCCA-202 - ENSP00000365463 [100%]
 

Other gene match

    
ANTIGEN VIEW
PCCA-001
PCCA-201
PCCA-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.